BIOARRAY™ 5-hmC methylation kit

• Single tube reaction format
• Suitable for analysis by a variety of downstream applications
• Flexible setup for custom assay design

The BIOARRAY™ 5-hmC methylation kit allows for sequence specific detection of 5-hydroxymethylcytosine within DNA using a simple and efficient glucosylation reaction procedure.

5-Hydroxymethylcytosine (5hmC), also known as the “6^th base”, is a newly discovered epigenetic modification. Although it was first identified in bacteriophages, its role was thought to be limited to protecting the phage genome from host induced restriction endonucleases. Interestingly, 5-hydroxymethylcytosine has recently been found in embryonic stem cells, the brain, as well as numerous other organs. Even though its presence has been confirmed, its biological role remains elusive. It has been proposed that 5-hydroxymethylcytosine may play a role in DNA demethylation (5-methylcytosine) or be involved in another layer of gene expression regulation.