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Figure A: Hybridomas producing MAbs to Bim (peptide) were identified using clones of the IL-3-dependent mouse myeloid cell line FDC-P1 stably expressing Bcl-2 alone, or Bcl-2 plus mouse BimL mixed at a 1:1 ratio. Transfected cells were fixed, permeabilized and stained with the rat anti-Bim MAb (clone 10B12) (Prod. No. ALX-804-528), the mouse anti-Glu-Glu (EE) MAb (positive control) or with secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.
Figure B: Western blotting with anti-Bim MAb (clone 10B12) (Prod. No. ALX-804-528) recognizes all known isforms of Bim. Western blotting of lysates of 293T cells cells transfected with EE-tagged forms of mouse or HA tagged forms of human Bim. Blots were probed with anti-Bim MAb (clone 10B12) (Prod. No. ALX-804-528)
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RUO – Research Use Only |
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