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BCMA (human):Fc (human), (recombinant)

ALX-522-026

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BCMA (human):Fc (human), (recombinant) Schematic structure — Schematic structure of human rhBCMA:Fc.

BCMA (human):Fc (human), (recombinant) Flow Cytometry — Figure: Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (2). Control (1) are untransfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.Method: 293 cells (5 x 10⁵) expressing surface BAFF vector were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 4µg/ml of BCMA:Fc. After washing in FACS buffer, PE-conjugated antibody to human IgG (SBA) was added and cells were analyzed by flow cytometry.

BCMA (human):Fc (human), (recombinant) Schematic structure — Schematic structure of human rhBCMA:Fc.

BCMA (human):Fc (human), (recombinant) Flow Cytometry — Figure: Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (2). Control (1) are untransfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.Method: 293 cells (5 x 105) expressing surface BAFF vector were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 4µg/ml of BCMA:Fc. After washing in FACS buffer, PE-conjugated antibody to human IgG (SBA) was added and cells were analyzed by flow cytometry.

Product Details

Alternative Name	TNFRSF 17, B cell maturation protein, CD269
Application Notes	ELISA: binds to mouse BAFF). Flow Cytometry: Detection of membrane-bound mouse BAFF and human APRIL in combination with fluorescently labelled Ab to IgG1.
Concentration	1mg/ml after reconstitution.
Formulation	Lyophilized. Contains PBS.
MW	~40kDa (SDS-PAGE).
Purity	≥95% (SDS-PAGE)
Reconstitution	Reconstitute with 50µl of sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Source	Produced in HEK 293 cells. The extracellular domain of human BCMA (aa 2-54) is fused at the C-terminus to the Fc portion of human IgG1.
Specificity	Binds human and mouse BAFF and APRIL.
Technical Info / Product Notes	Historical data shows that it blocks the binding of BAFF and APRIL to their receptors BCMA and TACI, inhibiting BAFF- and APRIL-mediated B cell activation.
UniProt ID	Q02223

Handling & Storage

Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Biomarkers of Efficacy and Safety of the Academic BCMA-CART ARI0002h for the Treatment of Refractory Multiple Myeloma: Oliver-Caldés, A., Español-Rego, M., et al.; Clin. Cancer Res. 30, 2085 (2024), Abstract
T cells isolated from G-CSF-treated multiple myeloma patients are suitable for the generation of BCMA-directed CAR-T cells: A.M. Battram, et al.; Mol. Ther. Methods Clin. Dev. 26, 207 (2022), Abstract
Long-Lived Plasma Cells from Human Small Intestine Biopsies Secrete Immunoglobulins for Many Weeks In Vitro: L. Mesin, et al.; J. Immunol. 187, 2867 (2011), Abstract
Blocking the APRIL circuit enhances acute myeloid leukemia cell chemosensitivity: D. Bonci, et al.; Haematologica 93, 1899 (2008), Abstract — Full Text
Essential role of antigen-presenting cell-derived BAFF for antibody responses: F. Bergamin, et al.; Eur. J. Immunol. 37, 3100 (2007), Abstract
Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha: M. Nishio, et al.; Blood 106, 1012 (2005), Abstract — Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004), Abstract
Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival: A.J. Novak, et al.; Blood 100, 2973 (2002), Abstract — Full Text
Maturation of marginal zone and follicular B cells requires B cell activating factor of the tumor necrosis factor family and is independent of B cell maturation antigen: P. Schneider, et al.; J. Exp. Med. 194, 1691 (2001), Abstract
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol. 167, 6225 (2001), Abstract

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Purity	≥90% (SDS-PAGE)
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