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BAFF (soluble) (mouse), (recombinant)

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Alternative Name	BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257
Application Notes	ELISA: binds to BAFF receptor at 1-100 ng/ml.
Concentration	0.1mg/ml after reconstitution.
Endotoxin Content	<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
Formulation	Lyophilized. Contains PBS.
Gene/Protein Identifier	A000383 (UCSD Signaling Gateway ID)
MW	~17kDa (SDS-PAGE).
Purity	≥90% (SDS-PAGE)
Reconstitution	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein.
Source	Produced in HEK 293 cells. The extracellular domain of mouse BAFF (aa 127-309) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG^®-tag.
Specificity	Binds to human (weak) and mouse BCMA, TACI and BAFF-R.
Technical Info / Product Notes	Historical data has shown that BAFF mediates splenocyte survival. FLAG is a registered trademark of Sigma-Aldrich Co.
UniProt ID	Q9WU72

Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Deleting the BAFF receptor TACI protects against systemic lupus erythematosus without extensive reduction of B cell numbers: W.A. Figgett, et al.; J. Autoimmun. 61, 9 (2015), Application(s): In Vitro Cultures, Abstract
BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med. 202, 1363 (2005), Abstract
Act1, a negative regulator in CD40- and BAFF-mediated B cell survival: Y. Qian, et al.; Immunity 21, 575 (2004), Abstract

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