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Figure: rhBAFF-R:Fc inhibits rhsBAFF-mediated activation of freshly isolated mouse splenocytes. Concentration of rhBAFF-R:Fc required to achieve complete inhibition depends on the concentration of rhsBAFF (Prod. No. ALX-522-025) used. A ratio (rhsBAFF:rhBAFF-R:Fc) 1:50 and higher is recommended.Method: On day 0 splenocytes were isolated from a freshly collected C57B16 spleen. An aliquot of the splenocytes was analyzed on FACS, and the B-cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media only, with the indicated concentration of rhsBAFF (Prod. No. ALX-522-025) alone or in the presence of excess rhsBAFF-R:Fc (+5µg/ml). After 3 days in culture

Product Details
Alternative Name |
TNFRSF 13C, BR3, BlySR3, CD268 |
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Application Notes |
ELISA: binds to BAFF. |
Concentration |
1mg/ml (after reconstitution) |
Endotoxin Content |
<0.1EU/µg purified protein (LAL test; Bio Whittaker). |
Formulation |
Lyophilized. Contains PBS. |
MW |
~40kDa (SDS-PAGE) |
Purity |
≥95% (SDS-PAGE) |
Reconstitution |
Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
Source |
Produced in HEK 293 cells. The extracellular domain of human BAFF-R (aa 2-71) is fused at the C-terminus to the Fc portion of human IgG1. |
Specificity |
Binds human and mouse BAFF. |
Technical Info / Product Notes |
Historical results have shown that BAFF-R inhibits rhsBAFF mediated splenocyte survival. |
UniProt ID |
Q96RJ3 |
Handling & Storage
Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. |
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Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Role of B Cell-Activating Factor in Fibrosis Progression in a Murine Model of Non-Alcoholic Steatohepatitis: K.K. Okada, et al.; Int. J. Mol. Sci. 24, 2509 (2023), Abstract
Related Products
Control:Fc fusion protein (human), (recombinant)
ALX-203-004
Negative control for Fc chimeric proteins

Alternative Name | Thy-1 (RLE mutant) |
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Purity | ≥90% (SDS-PAGE) |
Source | Produced in HEK 293 cells. The extracellular domain of mutated hThy-1, hThy-1 (RLE) (aa 20-130), is fused to the Fc portion of human IgG1. The integrin binding sequence RLD of hThy-1 has been changed to RLE abolishing the binding of integrins. |
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