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This antibody is covered by our Worry-Free Guarantee.

Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1µg/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.”””,BAFF (mouse) monoclonal antibody (5A8) Flow Cytometry,BAFF (mouse) monoclonal antibody (5A8) Flow Cytometry,yes
ALX-804-192,804-192.gif,http://static.enzolifesciences.com/fileadmin/files/image/804-192.gif,Figure: Photomicrographs of thymus from a mouse treated with hydrocortisone (left) and of a control treated animal (right). Sections of formalin-fixed tissues were heated in formamide

Product Details
Alternative Name |
B cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257 |
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Application |
ELISA, Flow Cytometry, WB |
Application Notes |
Excellent for Flow Cytometry. |
Clone |
5A8 |
Formulation |
Liquid. In PBS containing 0.02% sodium azide. |
Host |
Rat |
Immunogen |
Rat RBL cells expressing mouse BAFF. |
Isotype |
IgG1 |
Recommendation Dilutions/Conditions |
ELISA (capture)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Source |
Purified from ascites. |
Species Reactivity |
Mouse |
UniProt ID |
Q9WU72 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. |
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Handling |
Avoid freeze/thaw cycles. |
Short Term Storage |
+4°C |
Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- No interactions between heparin and atacicept, an antagonist of B cell survival cytokines.: Kowalczyk-Quintas, C., Willen, D., et al.; Br. J. Pharmacol. 176, 4019 (2019), Reactant(s): Human, Abstract
- TACI, unlike BAFF-R, is solely activated by oligomeric BAFF and APRIL to support survival of activated B cells and plasmablasts: C. Bossen, et al.; Blood 111, 1004 (2008), Abstract
- TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004), Abstract
Datasheet, Manuals, SDS & CofA
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Certificate of Analysis
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SDS
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