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Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1µg/ml of MAb to BAFF (mouse) (1C9). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
| Alternative Name | B cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257 |
|---|---|
| Application | ELISA, Flow Cytometry |
| Host | Rat |
| Isotype | IgG1 |
| Species Reactivity | Mouse |