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ATP (Energy) Regeneration Solution

BML-EW9810

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This solution contains MgCl₂, ATP, and ATP regenerating enzymes to recycle ATP hydrolysis products, (AMP, ADP) to ATP. An ideal energy source for conjugation/degradation assays (ubiquitin, proteasome, SUMO, etc.) particularly those that are preformed with crude lysates/impure reagents that may contain ATP-depleting activities.

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Product Details

Formulation	Liquid. In 50mM TRIS-Cl, pH 7.5.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. It is a 10x solution.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Amplification-Free Quantification of Endogenous Mitochondrial DNA Copy Number Using Solid-State Nanopores: Pal, S., Huttner, D., et al.; ACS Nano 19, 11390 (2025), Abstract
MARCH2, a Novel Oncogene-regulated SNAIL E3 Ligase, Suppresses Triple-negative Breast Cancer Metastases: Ito, K., Harada, I., et al.; Cancer Res. Commun. 4, 946 (2024), Abstract
ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade: Liu, X., Cen, X., et al.; Nat. Commun. 14, 4066 (2023), Abstract
Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis: Y. Fan, et al.; Cell Death Differ. , (2022), Abstract
A dimer-monomer switch controls CHIP-dependent substrate ubiquitylation and processing: V. Balaji, et al.; Mol. Cell 82, 3239 (2022), Abstract
Reduction in PA28αβ activation in HD mouse brain correlates to increased mHTT aggregation in cell models: K.W. Geijtenbeek, et al.; PLoS One 17, e0278130 (2022), Abstract
Enterovirus 3C Protease Cleaves TRIM7 To Dampen Its Antiviral Activity: Fan, W., McDougal, M. B., et al.; J. Virol. 96, e0133222 (2022), Abstract
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation: Y. Wu, et al.; Nat. Commun. 12, 2346 (2021), Abstract
Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation: M. Ranes, et al.; Mol. Cell 81, 3246 (2021), Abstract
DAXX represents a new type of protein-folding enabler: Huang, L., Agrawal, T., et al.; Nature 597, 132 (2021), Abstract

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ATP (Energy) Regeneration Solution

BML-EW9810

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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