Arg⁸-Vasopressin ELISA kit

• High sensitivity, detecting as little as 2.84 pg/ml
• Highly specific with negligible reactivity with oxytocin
• Simple and easy-to-use liquid color-coded reagents reduces error
• Fully quantitative results that surpass semi-quantitative Western blot analysis

The Arg⁸-Vasopressin ELISA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour. Absorbance is read at 450 nm.

Arginine vasopressin (AVP) is a nonapeptide hormone produced in magnocellular neurons of the hypothalamus, which functions as an antidiuretic in the kidney and systemically as a regulator of vasoconstriction, blood volume, and blood pressure. AVP acts by binding to a set of specific seven transmembrane domain-containing G-protein coupled receptors (GPCR) classified into three groups: V1a (V1), V1b (V3), and V2. V1b receptors stimulate adrenocorticotropic hormone (ACTH) release in the anterior pituitary, but may also participate in corticotropin-releasing hormone (CRH) release by the hypothalamus. The V1b receptor signals through coupling of Gq/11 alpha subunits, activating phospholipase C and release of intracellular Ca²⁺ via phosphoinositide second messengers. V1b receptor overexpression is a common marker for ACTH-secreting corticotropic adenomas. V2 receptors in the collecting ducts of the kidneys stimulate adenylyl cyclase activity, regulating the reabsorption of water via aquaporin channels.