AR Bioassay for Environmental Sample Monitoring

The principal application of this assay is to functionally screen processed water samples, with the aim of quantifying the bioactivities, if any, of contaminating endocrine disrupting chemical (EDC) entities. This assay utilizes proprietary mammalian cells expressing the native Androgen Receptor (AR; NR3C4), a ligand-dependent transcription factor. The cells also include the luciferase reporter gene functionally linked to an AR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in total AR activity.