APF LB Broth (Luria) (single packs)

LB Broth has been the venerable culture medium formulation used by researchers for over 50 years. First introduced in 1955, LB Broth has three different variations, designated as LB Miller, LB Lennox and LB Luria after the researchers who developed the formulations. LB is a simple medium composed of three ingredients: yeast extract, sodium chloride and casein hydrolysate. In recent years, the use of animal-derived raw materials has become a concern as a source of harmful contaminants to manufacturers of biopharmaceutical products. Initially, this concern was confined to the manufacturing end of the industry. As the regulatory environment has evolved in regard to the use of animal-derived materials, greater restrictions are being placed at earlier stages of product development. The consequence is a pressing need for media formulations which are not made from animal sources. To address this, AthenaES has developed a non-animal protein hydrolysate to replace the casein hydrolysate. An obvious choice for the casein replacement would be plant-derived hydrolysates, however, no one plant protein source has the same amino acid composition as casein. Therefore, the direct replacement of casein hydrolysate with a plant protein hydrolysate would not yield a medium with the same nutritional composition. To overcome this limitation, AthenaES has designed a blended plant-based protein hydrolysate that matches casein hydrolysate in composition and in performance.

APF LB Broths are superb media for the cultivation of E. coli, Salmonella, Pseudomonas, Bacillus and other eubacteria species.