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Figure: Western blot analysis with anti-Apaf-1 MAb clone 13F11 on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1- mice.

EndoG and Bnip3 are most highly expressed in rat heart, and are located to cardiac myocytes thorough development and during ischemia.a) Western blot analysis of EndoG, using a new anti-EndoG antibody (AntibodyBcn, BCN4778) and Bnip3 protein expression in total protein extracts from different adult rat tissues. EndoG is detected as a single band (∼27 kDa) and Bnip3 is detected as a group of bands around the 26 kDa mark (asterisk), in agreement with the information provided by the manufacturer. Both proteins are expressed mainly in the heart and skeletal muscle. Detection of EndoG and Bnip3 was performed in two different sets of independent samples with similar results. b) Western blot detection of EndoG, Bnip3 and proteins involved in caspase-dependent cell death in total protein extracts of ventricles from rats of different ages ranging from embryonic day 20 to adulthood. A representative image is shown from three independent experiments. c) Neonatal cardiomyocytes were treated with ischemia and the abundance of EndoG, Bnip3 and proteins involved in the caspase-dependent pathway was analyzed by Western blot in extracts obtained at different time points. Primary heart fibroblasts and neonatal liver samples were added for comparison. A representative image is shown from three independent experiments.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: EndoG links Bnip3-induced mitochondrial damage and caspase-independent DNA fragmentation in ischemic cardiomyocytes. PLoS One (2011)



Product Details
Alternative Name |
Apoptosis protease-activating factor 1 |
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Application |
ICC, IP, WB |
Clone |
13F11 |
Crossreactivity |
Does not cross-react with human Apaf-1. |
Formulation |
Liquid. In PBS containing 0.02% sodium azide. |
Host |
Rat |
Immunogen |
Recombinant mouse Apaf-1 (apoptosis protease-activating factor 1) (aa 1-97) containing the N-terminal CARD domain. |
Isotype |
IgG2a |
Purity Detail |
Protein G-affinity purified. |
Species Reactivity |
Mouse, Rat |
Specificity |
Recognizes the CARD domain of Apaf-1. |
UniProt ID |
O88879 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Handling |
Avoid freeze/thaw cycles. |
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Short Term Storage |
+4°C |
Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- EndoG links Bnip3-induced mitochondrial damage and caspase-independent DNA fragmentation in ischemic cardiomyocytes.: Cook, S. A., Zhang, J., et al.; PLoS One 6, e17998 (2011), Application(s): WB, Abstract
- Inhibition of apoptosome formation by suppression of Hsp90beta phosphorylation in tyrosine kinase-induced leukemias: M. Kurokawa, et al.; Mol. Cell Biol. 28, 5494 (2008), Abstract — Full Text
- Essential postmitochondrial function of p53 uncovered in DNA damage-induced apoptosis in neurons: A.E. Vaughn & M. Deshmukh; Cell Death Differ. 14, 973 (2007), Abstract — Full Text
- Decreased apoptosome activity with neuronal differentiation sets the threshold for strict IAP regulation of apoptosis: K.M. Wright, et al.; J. Cell Biol. 167, 303 (2004), Abstract — Full Text
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