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Apaf-1 (human) monoclonal antibody (2E12)

ALX-804-348

9 citations

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This antibody is covered by our Worry-Free Guarantee.

Apaf-1 (human) monoclonal antibody (2E12) Western blot — **Figure:** Western blot analysis with anti-Apaf-1 (human) MAb (clone 2E12) revealing endogenous Apaf-1. Lysates from 10⁶ HeLa, MCF-7, 293T, COS, Jurkat, and activated primary human T cells were probed with clone 2E12. COS (monkey) cells were used as a negative control.

Product Details

Alternative Name	Apoptosis protease-activating factor 1
Application	ELISA, ICC, IHC (FS), IP, WB
Clone	2E12
Crossreactivity	Does not cross-react with mouse or rat Apaf-1.
Formulation	Liquid. In PBS containing 0.02% sodium azide.
Host	Rat
Immunogen	Recombinant human Apaf-1 (apoptosis protease-activating factor 1) N-terminal CARD and CED-4 homologous domains (aa 1-464) .
Isotype	IgG2a
Purity Detail	Protein G-affinity purified.
Recommendation Dilutions/Conditions	Immunohistochemistry (EM on frozen sections; for details see G. Hausmann, et al., below.)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Human
Specificity	Recognizes the CARD domain of Apaf-1.
UniProt ID	O14727
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop.: Leslie, P. L., Franklin, D. A., et al.; Cell Rep. 24, 1484 (2018), Reactant(s): Human, Abstract
A network of substrates of the E3 ubiquitin ligases MDM2 and HUWE1 control apoptosis independently of p53: Kurokawa, M., Kim, J., et al.; Sci. Signal. 6, ra32 (2013), Abstract
FOXO transcription factors control E2F1 transcriptional specificity and apoptotic function: Shats, I., Gatza, M. L., et al.; Cancer Res. 73, 6056 (2013), Abstract
Rsk-mediated phosphorylation and 14-3-3ɛ binding of Apaf-1 suppresses cytochrome c-induced apoptosis: Kim, J., Parrish, A. B., et al.; EMBO J. 31, 1279 (2012), Abstract
BOK and NOXA Are Essential Mediators of p53-dependent Apoptosis: A.G. Yakovlev, et al.; J. Biol. Chem. 279, 28367 (2004), Abstract
Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein: D.H. Cho, et al.; J. Biol. Chem. 279, 39942 (2004), Abstract — Full Text
Anti-Fas antibody-induced apoptosis and its signal transduction in human gastric carcinoma cell lines: K. Adachi, et al.; Int. J. Oncol. 23, 713 (2003), Abstract
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000), Abstract — Full Text
Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1: K. Moriishi, et al.; PNAS 96, 9683 (1999), Abstract — Full Text

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Apaf-1 (human) monoclonal antibody (2E12)

ALX-804-348

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Handling & Storage

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