Antigen Retrieval Reagent, pH 9 (10X)

Manuals And Inserts

• Datasheet

Specific Protocol

Suggested Protocol:
 

1. Deparaffinize and rehydrate tissue sections.
2. Prepare a 1X Antigen Retrieval Solution by mixing 10 mL of Antigen Retrieval Reagent, pH 9 (10X) with 90 mL of deionized water to make 100 mL of solution.
3. Fill a coplin jar with sufficient Antigen Retrieval Solution (see Step 2) to cover the tissue sections on the slides.
4. Pre-heat steamer or water bath to 95-100°C.
5. Place coplin jar in steamer or water bath.
6. Place deparaffinized slides (1-3 slides/jar) in the coplin jar and incubate for 20-40 min (optimal incubation time and temperature should be determined by the end user, and may vary with different antibodies and tissue types).
7. Remove coplin jar from the water bath and allow the slides to cool for 20 min to reach room temperature.
8. Wash slides in deionized water and then with wash buffer. Proceed with immunostaining.

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