Antifade mounting medium with DAPI (hardset)

• Inhibits photobleaching of fluorescent dyes and fluorescent proteins
• Hardening formulation
• Includes DAPI counterstain in formulation

Antifade mounting medium with DAPI (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain. The inclusion of DAPI allows for staining of the nucleus.

All fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the dye, the intensity and the time of illumination. Many products have been developed to prevent fading of fluorescent dyes. These products often work well for one or more dyes, but often cause problems, such as degradation of other dyes. Antifade mounting medium with DAPI (hardset) works well with many stains including fluorescein and rhodamine.