Antibody Blocker/Diluent

Manuals And Inserts

• Datasheet

Specific Protocol

Recommended protocol for IHC (immunohistochemistry)

1. Arrange slides of formalin fixed paraffin-embedded (FFPE) tissue sections in a slide holder.
2. Heat in drying oven at 55-60°C for 20 minutes.
3. Deparaffinize with xylene or xylene substitute for 10 minutes.
4. Follow with 100% ethanol for 6 minutes.
5. Rehydrate slides for one minute in each step - 90%, 70%, 50% ethanol and in distilled or deionized water.
6. Incubate the tissue with peroxidase block for 5 minutes.
7. Wash with PBST for 2 minutes.
8. Retrieve antigen with  Antigen Retrieval Reagent (Citrate, pH 6) or Antigen Retrieval Reagent (Tris-EDTA, pH 9) for 20 minutes at 99°C.
9. Wash for 5 minutes with PBST.
10. Wipe the excess liquid around the section on the glass slide and encircle the tissue section with a PAP pen.
11. Dry slide for 10 seconds.
12. Incubate sections with Antibody Buffer/Diluent for 10 minutes to block non-specific antibody binding.
13. Researcher needs to optimize the concentrations and incubation times for primary antibodies (recommended 20 - 30 minutes)
14. Wash for 5 min with PBST.
15. After adding primary antibody, use POLYVIEW^® PLUS Detection reagents and HIGHDEF^® Chromogens to visualize antigens.

Certificate of Analysis

Please enter the lot number as featured on the product label

Lot No. :

SDS

Enzo Life Science provides GHS Compliant SDS

• English (EU) SDS
• Spanish; Castilian (EU) SDS
• French (EU) SDS
• English (USA) SDS

If your language is not available please fill out the SDS request form