FITC-labelled recombinant human annexin V fusion protein (produced in E. coli) shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
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Figure A: FACS histogram for the fluorescence intensity measured in FL-1 (FITC) channel.
Jurkat cells were incubated for 2 or 6 hours with medium (untreated) or with 100ng/ml rhsFasL in the presence of 1µg/ml Enhancer for Ligands (rhsFasL Set, Prod. No. ALX-850-014).
Figure B: Comparison of ALEXIS and Competitor Annexin-V. Jurkat cells were incubated for 2 or 6 hours with medium (background) or with 100ng/ml rhs FasL in the presence of 1µg/ml Enhancer for Ligands (rhs FasL Set, Prod. No. 850-014). Cells were stained with Annexin-V-FITC (Prod. No. 209-256 and competitor”

Product Details
Application Notes |
Detection of apoptotic cells by flow cytometry. |
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Concentration |
1mg/ml |
Formulation |
Liquid. In PBS, pH 7.4, containing 0.02% sodium azide. |
MW |
~52kDa (SDS-PAGE). |
Purity |
≥90% |
Purity Detail |
Affinity purified. |
Quantity |
Sufficient for 100 or 300 tests. 1 test is equivalent to 1μg of the conjugated protein in an experimental volume of 0.4 ml. |
Source |
Produced in E.coli. |
Specificity |
Binds to phosphatidylserine (PS). |
UniProt ID |
P08758 |
Handling & Storage
Use/Stability |
Stable for at least one year after receipt when stored at +4°C. |
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Handling |
Protect from light. Do not freeze. |
Long Term Storage |
+4°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- RIPK1 and RIPK3 are positive prognosticators for cervical cancer patients and C2 ceramide can inhibit tumor cell proliferation in vitro: Vogelsang, T. L. R., Kast, V., et al.; Front. Oncol. 13, 1110939 (2023), Abstract
- A Role for H2O2 and TRPM2 in the Induction of Cell Death: Studies in KGN Cells: Hack, C. T., Buck, T., et al.; Antioxidants (Basel) 8, (2019), Application(s): FC/FACS, Abstract
- Dopamine induces apoptosis in APPswe-expressing Neuro2A cells following Pepstatin-sensitive proteolysis of APP in acid compartments: M. Cagnin, et al.; Brain Res. 1471, 102 (2012), Abstract
- Upregulation of RGS4 and downregulation of CPI-17 mediate inhibition of colonic muscle contraction by interleukin-1beta: W. Hu, et al.; Am. J. Physiol. Cell Physiol. 293, 1991 (2007), Abstract — Full Text
Related Products

Alternative Name | APO-1L, CD95L, CD178, TNFSF 6 |
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Purity | ≥95% (SDS-PAGE) |
Source | Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG®-tag. Glycosylation of rhsFasL is similar to that of natural human FasL. |

Purity | ≥89% (SDS-PAGE, HPLC) |
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Source | Produced in E. coli. |

Purity | ≥89% (SDS-PAGE, HPLC) |
---|---|
Source | Produced in E. coli. |

Purity | ≥89% (SDS-PAGE, HPLC) |
---|---|
Source | Produced in E. coli. |
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Specific Protocol
Flow Cytometry Protocol:
-Treat cells for induction of apoptosis; As a recommended positive control, incubate with FasL (Prod. No. ALX-522-001) for 6 hours.
-Wash cells in PBS.
-Resuspend 200,000 cells in 0.4ml binding buffer (10mM HEPES/NaOH, pH7.4, 140mM sodium cloride, 2.5mM calcium cloride, filtered with 0.2μm filter.
- Add 1μl Annexin V-FITC to a final concentration of 2.5μg/ml. Incubate for 10 minutes in the dark.
-Wash cells once in binding buffer and resuspend in 0.4ml binding buffer.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application.
Certificate of Analysis
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SDS
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