Annexin V (human), (recombinant) (FITC conjugate)

Manuals And Inserts

• Datasheet

Specific Protocol

Flow Cytometry Protocol:
- Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂; filtered through 0.2µm pore filter).
- Brief centrifugation of annexin V-FITC before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down.
- Resuspend cells in binding buffer; adjust cell density to 2-5x10⁵/ml
- Take 195µl cell suspension and add 5µl annexin V-FITC
- Mix and incubate 10 min. in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
- FACS analysis

Certificate of Analysis

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SDS

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