Annexin V-FITC apoptosis detection kit

Manuals And Inserts

• Manual
• Datasheet

Specific Protocol

Flow Cytometry Protocol:

1. Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water)
2. Brief centrifugation of annexin V-FITC before use is recommended
3. Wash cells in PBS by gentle shaking or pipetting up and down.
4. Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x10⁵/ml
5. Take 195µl cell suspension and add 5µl annexin V-FITC
6. Mix and incubate 10 minutes in the dark at room temperature
7. Wash cells 1x with PBS and resuspend in 190µl prediluted binding buffer
8. Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
9. FACS analysis

Certificate of Analysis

Please enter the lot number as featured on the product label

Lot No. :

SDS

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