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Annexin V-FITC apoptosis detection kit

ALX-850-020

22 citations

Datasheet
Manual
SDS
CofA

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Easy, quick assay to distinguish apoptotic cells vs necrotic cells

FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm).

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Annexin V-FITC apoptosis detection kit Flow Cytometry — Typical flow cytometry results. The Annexin V-FITC apoptosis detection kit allows the detection and discrimination between apoptotic and necrotic cells.

Product Details

Application	Flow Cytometry, Fluorescence microscopy, Fluorescent detection
Application Notes	For the detection of apoptotic cells by flow cytometry or microscopy
Contents	Annexin V-FITC: 100 tests/500µl; 300 tests/1.5ml;Liquid. In 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide. Binding Buffer: 50ml (4x concentrated)Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl_2. Propidium Iodide: 20µg/ml (100 tests: 1.6ml, 300 tests: 2 x 1.6ml).
Purity	≥98% (SDS-PAGE; HPLC)

Handling & Storage

Handling	After opening annexin V-FITC, aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles.
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival: Rose, M., Burgess, J. T., et al.; Sci. Rep. 15, 25492 (2025), Abstract
The expression and role of the Lem-D proteins Ankle2, Emerin, Lemd2, and TMPO in triple-negative breast cancer cell growth: Rose, M., Burgess, J. T., et al.; Front. Oncol. 14, 1222698 (2024), Abstract
Targeting the COMMD4-H2B protein complex in lung cancer: M. Tang, et al.; Br. J. Cancer 129, 2014 (2023), Abstract
Stillage Waste from Strawberry Spirit Production as a Source of Bioactive Compounds with Antioxidant and Antiproliferative Potential: C. Spagnuolo, et al.; Antioxidants 12, 421 (2023), Abstract
The fructose-bisphosphate, Aldolase A (ALDOA), facilitates DNA-PKcs and ATM kinase activity to regulate DNA double-strand break repair: Sobanski, T., Suraweera, A., et al.; Sci. Rep. 13, 15171 (2023), Abstract
Dinuclear doubly bridged phenoxido copper(II) complexes as efficient anticancer agents: S.S. Massoud, et al.; Eur. J. Med. Chem. 246, 114992 (2023), Abstract
New glycoconjugation strategies for Ruthenium(II) arene complexes via phosphane ligands and assessment of their antiproliferative activity: D. Iacopini, et al.; Bioorg. Chem. 126, 105901 (2022), Abstract
Nigrosporins B, a Potential Anti-Cervical Cancer Agent, Induces Apoptosis and Protective Autophagy in Human Cervical Cancer Ca Ski Cells Mediated by PI3K/AKT/mTOR Signaling Pathway: J. Zhang, et al.; Molecules 27, 2431 (2022), Abstract
LncRNA XIST sponges microRNA-448 to promote malignant behaviors of colorectal cancer cells via regulating GRHL2: Z. Yan, et al.; Funct. Integr. Genomics 22, 977 (2022), Abstract
Protective function of DJ-1/PARK7 in lipopolysaccharide and ventilator-induced acute lung injury: Amatullah, H., Maron-Gutierrez, T., et al.; Redox Biol. 38, 101796 (2021), Abstract

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84 citations

Application	Flow Cytometry, Fluorescence microscopy, Fluorescent detection

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Specific Protocol

Flow Cytometry Protocol:

Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water)
Brief centrifugation of annexin V-FITC before use is recommended
Wash cells in PBS by gentle shaking or pipetting up and down.
Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x10⁵/ml
Take 195µl cell suspension and add 5µl annexin V-FITC
Mix and incubate 10 minutes in the dark at room temperature
Wash cells 1x with PBS and resuspend in 190µl prediluted binding buffer
Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
FACS analysis

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