AMPIVIEW® Wnt5a (AS) Dig RNA Probes (Rat) Set

ISH (in situ hybridization)

AMPIVIEW^® Wnt5a (AS) Dig RNA probes have been designed to target rat Wnt5a, a signaling molecule expressed embryonically during development. In addition to various aspects of development, Wnt5a has been implicated in many different cancers, cardiovascular and neurodegenerative diseases.

AMPIVIEW^® Wnt5a (AS) Dig RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo’s DIGX^® anti-digoxigenin linker and nanopolymner-based detection systems, POLYVIEW^® PLUS, combined with HIGHDEF^® chromogens and counterstain to produce clear results that can be visualized with a light microscope.

2 x 1 mL AMPIVIEW^® Wnt5a (AS) Dig RNA Probes (Rat) (2µg/mL)
1 x 2 mL AMPIVIEW^® Hybridization Buffer (1X)

One of the key signaling pathways that are known to play a crucial role in regulating various aspects of neurogenesis is Wnt signaling. Wnt5a is a signaling molecule expressed embryonically during gastrulation in various developing body regions. The role of Wnt5a has been extensively explored in various aspects of development. Loss of Wnt5a results in cerebellar hypoplasia and depletion of GABAergic and glutamatergic neurons. In addition to development, Wnt5a is implicated in many different types of cancer and cardiovascular disease, such as atherosclerosis.

AMPIVIEW^® Wnt5a (AS) Dig RNA Probes (Rat) are optimized for detection of nestin RNA or RNA/DNA in FFPE tissues or cells with DIGX^® anti-digoxigenin linker, POLYVIEW^® PLUS detection reagents combined with HIGHDEF^® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW^® Hybridization Buffer (1X), included in this set.