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AMPIVIEW^® Ubiquitin (AS) Dig RNA Probes Set

ENZ-GEN125

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A digoxigenin-labeled RNA probe for the in situ hybridization (ISH) detection ubiquitin RNA or DNA/RNA in cells and tissues.

Validated on the Roche DISCOVERY ULTRA Platform

AMPIVIEW^® Ubiquitin (AS) Dig RNA Probes are digoxigenin-labeled RNA probes targeting ubiquitin nucleic acid (DNA/RNA or RNA) for in situ hybridization in tissues and cells. The probes are formulated in a buffered formamide solution with hybridization enhancers.

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AMPIVIEW Ubiquitin (AS) Dig RNA Probes Set Prostate tissue Ubiquitin Detection — Ubiquitin (red) detected in prostate tissue with AMPIVIEW® Ubiquitin (AS) Dig RNA probes, DIGX® anti-digoxigenin linker, POLYVIEW® PLUS AP Detection reagent combined with HIGHDEF® Red AP IHC chromogen and counterstained with HIGHDEF® Hematoxylin.

AMPIVIEW Ubiquitin (AS) Dig RNA Probes Set Prostate tissue no signal — No signal detected in prostate tissue with AMPIVIEW® NSP Dig RNA probes, DIGX® anti-digoxigenin linker, POLYVIEW® PLUS AP Detection reagent combined with HIGHDEF® Red AP IHC chromogen and counterstained with HIGHDEF® Hematoxylin.

Product Details

Application	ISH (in situ hybridization)
Application Notes	AMPIVIEW^® Ubiquitin (AS) Dig RNA probes have been designed to target ubiquitin in tissues and cells. AMPIVIEW^® Ubiquitin (AS) Dig RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo’s DIGX^® anti-digoxigenin linker and nanopolymner-based detection systems, POLYVIEW^® PLUS, combined with HIGHDEF^® chromogens and counterstain to produce clear results that can be visualized with a light microscope.
Contents	2 x 1 mL AMPIVIEW^® Ubiquitin (AS) Dig RNA Probes (2µg/mL) 1 x 2 mL AMPIVIEW^® Hybridization Buffer (1X)
Technical Info / Product Notes	Ubiquitin is a regulatory protein found in nearly all eukaryotic organism. It is highly conserved and virtually identical across all forms of life and ideal to use as a positive control. AMPIVIEW^® Ubiquitin (AS) Dig RNA Probes are optimized for detection of ubiquitin RNA or RNA/DNA in FFPE tissues or cells with DIGX^® anti-digoxigenin linker, POLYVIEW^®> PLUS detection reagents combined with HIGHDEF^® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW^® Hybridization Buffer (1X), included in this set. Protocol for the Roche DISCOVERY ULTRA Platform: Download the protocol

Handling & Storage

Use/Stability	Aliquot and store at -20°C or -80°C. Under these conditions, products are stable until its expiration dates.
Handling	Allow contents to warm up to room temperature prior to use.
Short Term Storage	-20°C
Long Term Storage	-80°C
Shipping	Dry Ice