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AMPIVIEW^® HPV 16/18 RNA Probes Set

ENZ-GEN146

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A mixture of digoxigenin-labeled RNA probes for the detection of HPV 16/18 for in situ hybridization

AMPIVIEW^® RNA probes are uniquely designed with the precision of targeted, sequence-specific RNA probes, powered by Enzo’s LoopRNA™ ISH technology to deliver superior sensitivity. AMPIVIEW^® HPV 16/18 RNA probes is a mixture of digoxigenin-labeled probes targeting HPV type 16 and HPV type 18 for in situ hybridization. The probes are formulated in a buffered formamide solution with hybridization enhancers.

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Normal cervical tissue is negative for HPV 16/18. No signal detected with AMPIVIEW™ HPV 16/18 (ENZ-GEN146)

HPV infected cervical tissue is positive for HPV 16/18 (blue) detected with AMPIVIEW™ HPV 16/18 (ENZ-GEN146)

Product Details

Application	ISH (in situ hybridization)
Application Notes	AMPIVIEW^® HPV 16/18 RNA probes have been designed to target the human papillomavirus (HPV) type 16 and type 18 for its detection in tissues and cells. AMPIVIEW^® HPV16/18 RNA probes have been labeled with digoxigenin and have been optimized to produce clear results with Enzo’s nanopolymer-based detection system, POLYVIEW^® Plus, DIGX^® anti-digoxigenin linker and, high-quality chromogens and counterstains, HIGHDEF^® to produce clear results that can be visualized with a light microscope.
Contents	AMPIVIEW^® HPV 16/18 RNA Probes AMPIVIEW^® Hybridization Buffer (ENZ-ACC152)
Technical Info / Product Notes	AMPIVIEW^® HPV 16/18 RNA Probes are optimized for detection with either POLYVIEW^® PLUS detection reagents, HIGHDEF^® chromogens and counterstains (not inlcuded). Dilutions and concentration optimization can be done with AMPIVIEW^® hybridization buffer (ENZ-ACC152), included in this set.

Handling & Storage

Use/Stability	Upon receipt, aliquot and store at -20ºC. For long-term storage, you can store at -80ºC. Under these conditions, these products are stable until the expiration date.
Handling	Allow contents to warm up to room temperature prior to use.
Short Term Storage	-20°C
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Single cell transcriptomic analysis of HPV16-infected epithelium identifies a keratinocyte subpopulation implicated in cancer: M.C. Bedard, et al.; Nat. Commun. 14, 1975 (2023), Abstract
Recent improvements in immunohistochemistry and in situ hybridization: Gerard J. Nuovo, Jack Coleman, Maurizio Mauro; In Situ Molecular Pathology and Co-Expression Analyses 2nd Edition, 213-233 (2020), Book Chapter, Abstract
Recent improvements in in situ hybridization for the detection of HPV infections in clinical samples: G. La Rocca, J. Coleman, E. Rabbani, G. Palermo, F. Palermo, M. Mauro; World Cancer Research Journal WCRJ 2020; 7: e1542, (2020), Abstract