AMPIVIEW® GAPDH and NSP Dig Control Probes Kit

ISH (in situ hybridization)

AMPIVIEW^® GAPDH and NSP Dig Control Probes Kit contains AMPIVIEW^® GAPDH (AS) Dig RNA probes (positive control) and NSP Dig RNA probes (negative control) for in situ hybridization in tissues and cells. AMPIVIEW^® GAPDH (AS) Dig RNA probes have been designed to target human glyceraldehyde-3- phosphate dehydrogenase (GAPDH) in tissues and cells. AMPIVIEW^® NSP Dig RNA probes have been designed with a non-specific sequence to use as a negative control.  Both probes have a digoxigenin label and have been optimized to produce clear results with Enzo’s DIGX^® anti-digoxigenin linker and nanopolymner-based detection systems such as POLYVIEW^® PLUS, combined with HIGHDEF^® chromogens and counterstain to produce clear results that can be visualized with a light microscope.

2 x 1 mL AMPIVIEW^® GAPDH (AS) Dig RNA Probes (2µg/mL)
2 x 1 mL AMPIVIEW^® NSP Dig RNA Probes (RTU)
1 x 2 mL AMPIVIEW^® Hybridization Buffer (1X)

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic and key regulatory enzyme and most commonly used as a housekeeping gene.

AMPIVIEW^® GAPDH (AS) Dig RNA Probes are optimized for detection of GAPDH RNA or RNA/DNA in FFPE tissues or cells. AMPIVIEW^® NSP Dig RNA Probes are ready-to-use probes that have been designed with a non-specific sequence. Both probes can be detected with DIGX^® anti-digoxigenin linker, POLYVIEW^® PLUS detection reagents combined with HIGHDEF^® chromogens and counterstains (linker and detection solutions not included). Dilutions and concentration optimization can be done with AMPIVIEW^® Hybridization Buffer (1X), included in this kit.