AMPIPROBE^® UP/Mg/Mh Extraction Controls

• Compatible witrh manual and automated DNA extraction methods
• Compatible with most open qPCR platforms

The AMPIPROBE^® UP/Mg/Mh Extraction Controls are designed to be used during the genomic DNA extraction of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium, and Mycoplasma hominis.
All performance specifications must be validated by end-user and will vary based on chosen extraction methods. Eluent from extraction can be used as extraction control for AMPIPROBE^® UP/Mg/Mh Assay kit (Prod. No. ENZ-GEN209).
Plasmids in Extraction Controls are present at higher concentrations compared to assay controls included in the qPCR kit.  Extra care should be taken to avoid contamination.

Ureaplasma and Mycoplasma, which belong to the same Mycoplasmataceae family and Mollicutes class, are the smallest self-replicating organisms and are characterized by their lack of a cell wall. These characteristics and limited biosynthetic capabilities contribute to the parasitic nature of Ureaplasma and Mycoplasma.
The class of pathogens is present in healthy individuals but has been associated with many adverse conditions affecting the reproductive tract. Mycoplasma genitalium is associated with urethritis, cervical inflammation, and pelvic inflammatory disease. M. hominis is often present concurrently with Ureaplasma species and is associated with a variety of conditions ranging from pelvic inflammatory diseases, chorioamnionitis, postpartum endometritis bacterial vaginosis, arthritis, osteoarthritis, wound infections, and several conditions in neonates.
Ureaplasma and Mycoplasma, especially in combination with other conditions such as bacterial vaginosis or cervical incompetence, have been associated with adverse pregnancy outcomes, such as chorioamnionitis, spontaneous preterm labor and preterm premature rupture of membranes.
The AMPIPROBE^® UMM Assay provides rapid and accurate results for the qualitative detection of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium, and Mycoplasma hominis DNA in a user-supplied sample of interest.