AMPINEXT™ Post-Bisulfite DNA Library Preparation Kit (Illumina)

• Allows bisulfite-converted DNA to be used directly for ligation.
• Fast 5-hour procedure, from input starting material to library amplification.
• Gel-free size selection/purification
• High sensitivty and efficiency — direct ligation of adapter to bisulfite-converted DNA fragments.
• Comprehensive set of components to accommodate each step of DNA library preparation.
• Ultra HiFi amplification enables achievement of reproducibly high yields of DNA libraries.

The AMPINEXT™ Post-Bisulfite DNA Library Preparation Kit is a complete set of optimized reagents to prepare a DNA library — after successful bisulfite conversion — for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representation bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation.