AMPINEXT™ High-Sensitivity Bisulfite-Seq Kit (Illumina)

• Innovative method – Allows for simultaneous bisulfite conversion and size-appropriate DNA fragmentation. .
• Fast and streamlined procedure – Ready-to-use library DNA within 6 hours and 30 minutes.
• Complete conversion –  Converts unmethylated cytosine into uracil (>99%).
• High sensitivity and efficiency – Innovative adaptor ligation of bisulfite DNA eliminates loss of fragments and selection bias.
• Extremely convenient – The kit contains all required components for each step of DNA library preparation,
• Minimal bias – Ultra HiFi amplification.
• Broad sample suitability – Starting materials can be genomic DNA isolated from various tissue/cell samples.

The AMPINEXT™ High-Sensitivity Bisulfite-Seq Kit (Illumina) is designed to bisulfite-convert DNA and prepare an Illumina-based library for bisulfite sequencing, all in one kit. Intended applications include whole genome (WGBS), oxidative (oxBS-seq), reduced representation (RRBS), and other bisulfite-next generation sequencing. The optimized protocol and components of the kit allow subnanogram amounts of DNA to be  bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction in less than 7 hours.