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AMPINEXT™ DNA Size Selection Kit

ENZ-GEN506

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A complete set of optimized reagents for quick removal of DNA fragments of <150 bps for library preparation in next generation sequencing applications.

Optimized fragment selection chemistries: Complete separation of DNA fragments according to size.
Fast and straightforward: Finished within 30 mins with no gels, columns or centrifugation are needed.
Efficient removal of primer-dimers: No need for further clean-up steps.
High recovery of targeted DNA fragments: Higher than 85% recovery of input DNA fragments.
Manual and automation friendly: Scalable for use in single tube or 96-well plate format.

The AMPINEXT™ DNA Size Selection Kit is a complete set of optimized reagents for quick removal of DNA fragments of <150 bps for library preparation in next generation sequencing applications. The kit utilizes magnetic bead technology and is suitable for Illumina, Life Technologies (SOLiD), Ion Torrent, and Roche/454 platforms. It can also be used for removing undesired larger DNA fragments by optimizing the bead to DNA volume ratio accordingly. The indicated number of reactions can be performed for a standard 50 µl solution input DNA sample.

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AMPINEXT DNA Size Selection Kit Schematic — Figure 1. Schematic Procedure for using the AMPINEXT DNA Size Selection Kit

Product Details

Application Notes	A complete set of optimized reagents for quick removal of DNA fragments of <150 bps for library preparation in next generation sequencing applications.
Assay Time	30 minutes
Contents	MQ Binding Beads Elution Solution 96-well plate
Technical Info / Product Notes	DNA size selection is necessary after DNA shearing as part of the library construction process for next generation sequencing, regardless of the platform used. Obtaining high recovery of selected DNA fragments is critical for the reduction of sequencing bias.

Handling & Storage

Use/Stability	Upon receipt: Store the following components at 4°C: MQ Binding Beads, Elution Solution. Store all other components at room temperature. The kit is stable for at least 6 months from the shipment date, when stored properly.
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Specific Protocol

This kit includes all reagents required at each step of the workflow for DNA size selection. The size of DNA fragments bound to the included MQ Binding Beads is based on the ratio of MQ beads to the DNA sample solution. Optimization of MQ bead ratio to input DNA, allows for the removal of larger or smaller DNA fragments and recovery of desired target size DNA fragments.

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