AMPINEXT™ 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)

• High sensitivity and efficiency: Use for non-barcoded (singleplexed) and barcoded (multiplexed) library preparation.
• Easy and streamlined procedure: The entire procedure can be finished in 6 hours.
• Complete conversion: Converts unmethylated cytosine into uracil at a level greater than 99.9%.
• Extremely convenient: The kit contains all the required components for each step of the RNA library preparation process.
• Minimal bias: Ultra HiFi amplification.
• Broad sample suitability: Starting materials can be total RNA isolated from various tissue/cell samples.

The AMPINEXT™ 5-mC RNA Bisulfite-Seq Easy Kit (Illumina) is a complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a “post-bisulfite” library preparation process for Illumina platform-based bisulfite sequencing, all in one kit. Intended applications include whole transcriptome RNA bisulfite sequencing and various other RNA bisulfite-based next generation sequencing techniques for 5-methylcytosine (5-mC) based RNA methylation analysis. The optimized protocol and components of the kit allow the RNA to be bisulfite converted and fragmented simultaneously, followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using low-nanogram quantities of bisulfite converted RNA.