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- 100x greater fidelity than Taq DNA polymerase
- Increased processivity (10-30s/kb)
- Amplification of challenging GC and AT-rich templates to over 17.5kb
- Hot start technology for maximized sensitivity and specificity
AMPIGENE® Hot Start High Fidelity Polymerase was engineered to improve DNA binding resulting in amplification of difficult targets, greater yields, and shorter extension times.
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AMPIGENE® Hot Start High Fidelity Polymerase Provides Enhanced Performance with GC-Rich Templates
AMPIGENE® Hot Start High Fidelity Polymerase exhibited superior performance with GC-rich template in comparison to the competitors’ product. The template used was 500 pb long with 69% GC content.
AMPIGENE® Hot Start High Fidelity Polymerase Provides Enhanced Performance with Difficult Templates
AMPIGENE® Hot Start High Fidelity Polymerase exhibited superior performance at different annealing temperatures (Ta1 = 68.5°C, Ta2 = 66.0°C, Ta3 = 63.0°C, Ta4 = 60.5°C) compared to the competitors’ product. AMPIGENE® Hot Start High Fidelity Polymerase generated cleaner bands and no primer dimers. Human gDNA, 30 ng (8333 copies) and 13500 bp long was used as template.
| Regulatory Status |
RUO – Research Use Only |
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