AMP’D^® HSP70 high sensitivity ELISA kit

• Ultra-sensitive measurement of Hsp70, detecting as little as 7 pg/ml
• Ability to detect both baseline and upregulated/stressed levels of Hsp70 in human, mouse and rat serum and plasma samples
• Negligible reactivity from similar Hsp70 family members (Hsc70/Hsp73, Grp78, DnaK, or Hsp71)
• High throughput format with results in 4.5 hours for up to 38 samples in duplicate
• Fully quantitative results that surpass semi-quantitative Western blot analysis

The AMP’D^® HSP70 high sensitivity ELISA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4.5 hours.

The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal  pathway.