AMP’D^® ELISA Signal Amplification Kit

• Designed to amplify sandwich/immunometric ELISA signal
• Increase ELISA sensitivity greater than 10-fold
• Flexibility to work with own targets
• Convenient one or five 96-well plate formats for high throughput analysis
• Easy-to-use, simple procedure with results in just 30 minutes

The AMP’D^® ELISA Signal Amplification  kit is designed to replace traditional alkaline phosphatase (AP) substrates, such as pNPP (p-Nitrophenyl phosphate), with a combination substrate and amplifier system that results in greater sensitivity when compared to a classic substrate enzyme linked-immunosorbent assay (ELISA).

In the AMP’D^® ELISA Signal Amplification kit, bound AP converts a substrate that is utilized in a second enzyme reaction system which is initiated by addition of the amplifier reagent.  It is this amplification step that allows for greater (amplified) color production at lower analyte concentrations resulting in an increase in assay sensitivity.