Ammonia Assay II

Ammonia is important both as a source of and a means of eliminating nitrogen in living systems. It is required for the synthesis of amino acids, as well as nucleotides and some lipids. Ammonia is typically formed through amino acid deamination. In the liver, ammonia is converted to the much less toxic urea and in birds and reptiles, into uric acid. AkrivisBio’s Ammonia Assay II is a simple, sensitive, reliable assay with a sensitivity below 1 nmole per 100 μl well. In the assay, ammonia is converted to indophenol, with a strong absorbance measured at 670 nm.