Aminoallyl-dUTP-XX – ATTO-425, S pack

Aminoallyl-dUTP-XX-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.
Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-ATTO425/ 80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-XX-ATTO425/ 70-50% dTTP (Nick Translation) Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.