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Aldolase C is a member of the class I fructose-biphosphate aldolase family. This protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1, 6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehydes respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain.
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IF analysis of ALDOC in Jurkat cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
IF analysis of ALDOC in U87MG cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
The cell line and tissue lysates (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with the ALDOC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: PC3 cell lysate, Lane 2: U87MG cell lysate, Lane 3: WiDr cell lysate, Lane 4: Jurkat cell lysate, Lane 5: Mouse brain tissue lysate.
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RUO – Research Use Only |
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