Akt monoclonal antibody (5C10)

Protein kinase B, PKB

ELISA, WB

5C10

Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Mouse

Synthetic peptide corresponding to C-terminal human Akt (protein kinase B; PKB) conjugated to KLH.

IgG1

Included. (Prod. No. 840-030-1)

Thiophilic adsorption and size exclusion chromatography purified.

Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitute with 1ml water (15 minutes at room temperature).

Dog, Human, Mouse, Rat

Recognizes a C-terminal epitope (aa 466-480) of phosphorylated and non-phosphorylated Akt isoforms (see Technical Note below).

Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBa), Akt2 (PKBb), and Akt3 (PKBg). Akt1 shares 81% and 83% amino acid identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms. Thus the phosphorylation site Ser⁴⁷³ of Akt1 corresponds to Ser⁴⁷⁴ in Akt2 and Ser⁴⁷² in Akt3 respectively.

Akt1: V⁴⁶¹DSERRPHFPQFS⁴⁷³YSASSTA⁴⁸⁰ UniProt ID P31749
Akt2: L⁴⁶¹LELDQRTHFPQFS⁴⁷⁴YSASIRE⁴⁸¹ UniProt ID P31751
Akt3: D⁴⁶¹NERRPHFPQFS⁴⁷²YSASGRE⁴⁷⁹ UniProt ID Q9Y243

Replacement for ADI-905-661

P31749 (Akt1), P31751 (Akt2), Q9Y243 (Akt3)

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