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Western blot analysis of AhR, pAb (Prod. No. BML-SA550): Lane 1: MW marker; Lane 2: Rat liver lysate; Lane 3: Mouse liver lysate.
AHR is an IFN Effector Gene and Interacts with HELZ2. RNAi-mediated knockdown of AHR with four unique siRNAs leads to weak but consistent rescue of DENV from IFN in HeLa cells (A) and Huh7.5.1 hepatocytes (B). (C) Knockdown of AHR by the four unique siRNAs from (A) was validated at the protein level using western blot (C) with quantification (D) and at the mRNA level using qRT PCR (E). (F) Co-immunoprecipitation of AHR with HELZ2 (H) and AHR (A) antibodies and isotype control (I) was performed in Huh7.5.1 cells treated with IFN 1,600 IU/ml × 24 h or mock, followed by immunoblot for AHR. Load controls (Ld) were run in parallel, and immunoblotted for AHR and actin (G). Co-immunoprecipitation was repeated in the setting of mock (M), IFN 1,600 IUml (I), or the AHR agonist FICZ 0.9 um (F) × 24 h, using HELZ2 and AHR antibodies for IP, followed by HELZ2 and AHR immunoblot. Load controls were stained for HELZ2, AHR, and actin. Ladder is indicated by L. *p < 0.05 compared to control.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: HELZ2 Is an IFN Effector Mediating Suppression of Dengue Virus. Front Microbiol (2017)
AHR is an IFN Effector Gene and Interacts with HELZ2. RNAi-mediated knockdown of AHR with four unique siRNAs leads to weak but consistent rescue of DENV from IFN in HeLa cells (A) and Huh7.5.1 hepatocytes (B). (C) Knockdown of AHR by the four unique siRNAs from (A) was validated at the protein level using western blot (C) with quantification (D) and at the mRNA level using qRT PCR (E). (F) Co-immunoprecipitation of AHR with HELZ2 (H) and AHR (A) antibodies and isotype control (I) was performed in Huh7.5.1 cells treated with IFN 1,600 IU/ml × 24 h or mock, followed by immunoblot for AHR. Load controls (Ld) were run in parallel, and immunoblotted for AHR and actin (G). Co-immunoprecipitation was repeated in the setting of mock (M), IFN 1,600 IUml (I), or the AHR agonist FICZ 0.9 um (F) × 24 h, using HELZ2 and AHR antibodies for IP, followed by HELZ2 and AHR immunoblot. Load controls were stained for HELZ2, AHR, and actin. Ladder is indicated by L. *p < 0.05 compared to control.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: HELZ2 Is an IFN Effector Mediating Suppression of Dengue Virus. Front Microbiol (2017)
| Regulatory Status |
RUO – Research Use Only |
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