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Control compound for rmsMEGACD40L® (Prod. No. ALX-522-120).
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Figure: ACRP30headless (mouse) does not bind to rhTNF-R1 and rhOX40:Fc in an ELISA assay.
Method: A 96 well ELISA plate was coated O/N at RT with 100 µl of 1 µg/ml rhTNF-R1:Fc (Prod. No. ALX-522-013) and 1 µg/ml rhOX40:Fc (Prod. No. ALX-522-022) in PBS. 100 µl of rmsACRP30headless at 8 µg/ml were mixed with 100 µl 0.5% FCS in PBS in the first well and a series of 1:1 dilutions was prepared for the following wells. Binding of rmsACRP30headless to the receptor:Fc was detected after 1 hr incubation at 37°C using a goat anti-human HRP conjugated antibody (Jackson lab; 1:1″””
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
MEGACD40L® Protein (soluble) (mouse), (recombinant)
ALX-522-120
High activity, high purity CD40L protein for co-stimulatory activation of an immune response
| Alternative Name | CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF5:ACRP30headless, gp39:ACRP30headless |
|---|---|
| Purity | ≥95% (SDS-PAGE) |
| Source | Produced in CHO cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
Last modified: May 29, 2024