Acetyltransferase activity kit

• Universal – suitable for any AcetylCoA-dependent acetyltransferase
• Robust – Z’ factor = 0.87
• Sensitive – fluorescent detection allows for both DNA and protein assays
• Higher throughput – homogeneous mix-and-read assay

The Acetyltransferase activity kit is a homogeneous mix-and-read fluorescent assay for the determination of any acetyl-CoA dependent acetyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.

Acetylation of proteins and drugs is a regulatory event that can result in the activation, inactivation, or detoxification of molecules including histones, kinases, transcription factors, anti-microbial agents, and neurotransmitters. While acetylation can be carried out by a variety of acetyltransferase enzymes using diverse substrates, their common cofactor or acetyl donor is acetyl-CoA.