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Caspase-1 substrate
Fluorogenic caspase-1 substrate with a Km=14µM. Ex.:340-360nm, Em.:440-460nm.Sequence is based on precursor interleukin-1β Asp116 cleavage site for caspase-1.
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Product Details
| Alternative Name |
Caspase-1 substrate (fluorogenic) |
|---|---|
| Appearance |
White to off-white powder. |
| Formula |
C33H39N5O10 |
| MW |
665.7 |
| Peptide Content |
75-95% |
| Purity |
≥98% (HPLC) |
| Sequence |
Ac-Tyr-Val-Ala-Asp-AMC (AMC = 7-Amino-4-methylcoumarin) |
| Solubility |
Soluble in DMSO. Dilute with distilled water or buffer, pH 7.0-7.5 (e. g. TRIS, HEPES). |
| Technical Info / Product Notes |
AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm. |
Handling & Storage
| Use/Stability |
As indicated on product label or CoA when stored as recommended. |
|---|---|
| Handling |
Protect from light. Keep cool and dry. |
| Long Term Storage |
-20°C |
| Shipping |
Ambient Temperature |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Cardioprotective properties of OMT-28, a synthetic analog of omega-3 epoxyeicosanoids: Kranrod, J., Konkel, A., et al.; J. Biol. Chem. 300, 107372 (2024), Abstract
- Cardioprotective Action of a Novel Synthetic 19,20-EDP Analog Is Sirt Dependent: Kranrod, J. W., Darwesh, A. M., et al.; J. Cardiovasc. Pharmacol. 83, 105 (2024), Abstract
- Characterization of heart disease in mucopolysaccharidosis type II mice: A.M.V. Tavares, et al.; Cardiovasc. Pathol. 67, 107575 (2023), Abstract
- Trimethylamine N-Oxide (TMAO) Mediates Increased Inflammation and Colonization of Bladder Epithelial Cells during a Uropathogenic E. coli Infection In Vitro: R. Wu, et al.; Pathogens 12, 523 (2023), Abstract
- Neutrophil membrane-coated nanoparticles exhibit increased antimicrobial activities in an anti-microbial resistant K. pneumonia infection model: J. Liu, et al.; Nanomedicine 48, 102640 (2023), Abstract
- Cardioprotective Effects of Oroxylum indicum Extract Against Doxorubicin and Cyclophosphamide-Induced Cardiotoxicity: S.R. Pondugula, et al.; Cardiovasc. Toxicol. 22, 67 (2022), Abstract
- Cardiomyocyte-specific disruption of soluble epoxide hydrolase limits inflammation to preserve cardiac function: Sosnowski, D. K., Jamieson, K. L., et al.; Am. J. Physiol. Heart Circ. Physiol. 323, H670 (2022), Abstract
- The structure of the human LACTB filament reveals the mechanisms of assembly and membrane binding: J.A. Bennett, et al.; PLoS Biol. 20, e3001899 (2022), Abstract
- β-hydroxybutyric acid attenuates oxidative stress and improves markers of mitochondrial function in the HT-22 hippocampal cell line: M. Majrashi, et al.; J. Integr. Neurosci. 20, 321 (2021), Abstract
- A Synthetic Epoxydocosapentaenoic Acid Analogue Ameliorates Cardiac Ischemia/Reperfusion Injury: The Involvement of the Sirtuin 3-NLRP3 Pathway: Darwesh, A. M., Bassiouni, W., et al.; Int. J. Mol. Sci. 21, (2020), Abstract
- Niclosamide activates the NLRP3 inflammasome by intracellular acidification and mitochondrial inhibition: Thi Tran, U., Kitami, T., et al.; Commun. Biol. 2, 2 (2019), Abstract
- Genetic Deletion or Pharmacological Inhibition of Soluble Epoxide Hydrolase Ameliorates Cardiac Ischemia/Reperfusion Injury by Attenuating NLRP3 Inflammasome Activation: Darwesh, A. M., Keshavarz-Bahaghighat, H., et al.; Int. J. Mol. Sci. 20, (2019), Abstract
- Clearance of intracellular Klebsiella pneumoniae infection using gentamicin-loaded nanoparticles: L. Jiang, et al.; J. Control Release 279, 316 (2018), Abstract
- Reperfusion therapy with recombinant human relaxin-2 (Serelaxin) attenuates myocardial infarct size and NLRP3 inflammasome following ischemia/reperfusion injury via eNOS-dependent mechanism: J.V. Raleigh, et al.; Cardiovasc Res 113, 609 (2017), Application(s): Caspase-1 activity in mouse heart homogenates, Abstract
- Design and application of a fluorogenic assay for monitoring inflammatory caspase activity: R. Ranganathan, et al.; Anal. Biochem. 543, 1 (2017), Abstract
- Temporal Changes in Caspase-1 and Caspase-8 Activities Following Brain Hypoxia With and Without Src kinase Inhibition in a Piglet Animal Model: D. Angelis, et al.; Neurochem. Res. 40, 2270 (2015), Abstract
- Interleukin-1 β converting enzyme: N.A. Thornberry; Meth. Enzymol. 244, 615 (1994), Abstract
- Crystal structure of the cysteine protease interleukin-1 β-converting enzyme: a (p20/p10)2 homodimer: N.P.C. Walker, et al.; Cell 78, 343 (1994), Abstract
- Interleukin 1 beta (IL-1 beta) processing in murine macrophages requires a structurally conserved homologue of human IL-1 beta converting enzyme: S.M. Molineaux, et al.; PNAS 90, 1809 (1993), Abstract
- Molecular cloning of the interleukin-1 beta converting enzyme: D.P. Cerretti, et al.; Science 256, 97 (1992), Abstract
- A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes: N.A. Thornberry, et al.; Nature 356, 768 (1992), Abstract
- IL-1-converting enzyme requires aspartic acid residues for processing of the IL-1 beta precursor at two distinct sites and does not cleave 31-kDa IL-1 alpha: A.D. Howard, et al.; J. Immunol. 147, 2964 (1991), Abstract
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