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Ac-WEHD-AMC

ALX-260-057

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Substrate for caspase-1, -4 and -5

Fluorogenic substrate for caspase-1 (K_m=4µM), -4 (K_m=31µM);and -5 (K_m=15µM). This fluorogenic caspase-1 substrate with an optimal tetrapeptide sequence is cleaved 50-fold more efficiently than Ac-YVAD-AMC (Prod. No. ALX-260-024).

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Product Details

Alternative Name	Caspase-1 substrate (fluorogenic), Caspase-5 substrate (fluorogenic), Caspase-4 substrate (fluorogenic)
Formula	C₃₈H₄₀N₈O₁₁
Formulation	Lyophilized.
MW	784.8
Peptide Content	70-90%
Purity	≥98% (HPLC)
Sequence	Ac-Trp-Glu-His-Asp-AMC (AMC=7-Amino-4-methylcoumarin)
Solubility	Soluble in DMSO (100mM, 75.5mg/ml). Dilute with distilled water or buffer.
Technical Info / Product Notes	AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light and moisture.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation: D.Y. Cao, et al.; Immunol. Lett. 244, 28 (2022), Abstract
Design and application of a fluorogenic assay for monitoring inflammatory caspase activity: R. Ranganathan, et al.; Anal. Biochem. 543, 1 (2017), Abstract
Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC6: N. Van Opdenbosch, et al.; Cell Rep. 21, 3427 (2017), Abstract — Full Text
Synthesis of a small molecule nitrosocysteine inhibitor to reduce the activity of Caspase-1: C.A. Shamblen & C.E. Karver; DePaul Discoveries 5, Article 5 (2016), Application(s): Caspase-1 inhibition assay, Full Text
CD 95-independent mechanisms of IL-2 deprivation-induced apoptosis in activated human lymphocytes: T. Hieronymus, et al.; Cell Death Differ. 7, 538 (2000), Abstract
Purification and catalytic properties of human caspase family members: M. Garcia-Calvo, et al.; Cell Death Differ. 6, 362 (1999), Application(s): Kinetic constants for cleavage of fluorescent substrates, Abstract
A combinatorial approach defines specificities of members of the caspase family and granzyme B: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract — Full Text

Related Products

Ac-YVAD-AMC

ALX-260-024

Caspase-1 substrate

22 citations

Purity	≥98% (HPLC)

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Ac-WEHD-AMC

ALX-260-057

Product Details

Handling & Storage

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