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Colorimetric substrate for caspase-6 (Km=30µM; Kcat/Km=168’000 M-1sec-1) and related cysteine proteases. Sequence is based on the lamin A site of cleavage. Cleavage of the substrate by a caspase releases pNA, which can be detected by its absorbance at 405nm (ε=10,500 M-1cm-1).
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Last modified: May 29, 2024