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Ac-LEVD-AFC

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Caspase-4 substrate

Fluorogenic substrate for caspase-4. Similar to the fluorogenic substrate Ac-LEVD-AMC (Prod. No. ALX-260-083); the AFC fluorophore is more sensitive.

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Alternative Name	Caspase-4 substrate (fluorogenic)
Appearance	White to off-white powder.
Formula	C₃₂H₄₀F₃N₅O₁₁
MW	727.7
Peptide Content	75-95%.
Purity	≥95% (HPLC)
Sequence	Ac-Leu-Glu-Val-Asp-AFC (AFC=7-Amido-4-trifluoromethylcoumarin)
Solubility	Soluble in DMSO; dilute with distilled water or buffer; also soluble in phosphate buffer, pH 7.5-8.0.
Technical Info / Product Notes	AFC has an excitation maximum of 400nm and an emission maximum of 505nm.

Use/Stability	As indicated on product label or CoA when stored as recommended. Dissolve only as much as needed at one time because of the possibility of decomposition in solution. Store solution at -20°C. Protect from condensation: when taking the sample out of the container, wait until it warms up to room temperature, then open it.
Handling	Keep cool and dry.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

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Caspase-4 substrate

Purity	≥99% (HPLC)

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