Ac-LEHD-pNA

Manuals And Inserts

• Datasheet

Specific Protocol

HEPES-Buffer: 100 mM HEPES, pH 7.5; 20% (v/v)
 glycerol; 5 mM DTT, 0.5 mM EDTA.

Substrate: Prepare 1 mM stock solution in DMSO.
- Induce apoptosis and prepare cell lysate or use recombinant caspase.
- Prepare reaction solution:
30 µl of substrate stock solution + 240 µl HEPES-Buffer + 30 µl of cell lysate.
- Incubate for 1 hour at 37 °C.
- Measure with microplate reader at 400nm.
- Suggested controls: Reaction mixture…
…without substrate.
 …with non-apoptotic cell lysate.
 …with apoptotic cell lysate and caspase inhibitor.

Certificate of Analysis

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