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Ac-IETD-AFC

ALX-260-110

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Caspase and granzyme substrate

Fluorogenic substrate for caspase-8/caspase-3 processing enzyme and granzyme B. Also a substrate for caspase-10. The IETD sequence is based on caspase-3 proenzyme cleavage site.

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Product Details

Alternative Name	Caspase-8 substrate (fluorogenic), Granzyme B substrate (fluorogenic)
Appearance	White to off-white powder.
Formula	C₃₁H₃₈F₃N₅O₁₂
MW	729.7
Purity	≥98% (HPLC)
Sequence	Ac-Ile-Glu-Thr-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin)
Solubility	Soluble in DMSO.
Technical Info / Product Notes	AFC has an excitation maximum of 400nm and an emission maximum of 505nm.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Keep cool and dry.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout: S. Devi, et al.; Cell Rep. 42, 112265 (2023), Abstract
Ubiquitination of interleukin-1α is associated with increased pro-inflammatory polarization of murine macrophages deficient in the E3 ligase ITCH: Lin, X., Zhang, H., et al.; J. Biol. Chem. 295, 11764 (2020), Abstract
Nimbolide reduces CD44 positive cell population and induces mitochondrial apoptosis in pancreatic cancer cells: S. Kumar, et al.; Cancer Lett. 413, 82 (2017), Abstract
Sub-lethal oxidative stress induces lysosome biogenesis via a lysosomal membrane permeabilization-cathepsin-caspase 3-transcription factor EB-dependent pathway: S.M. Leow, et al.; Oncotarget 8, 16170 (2017), Abstract — Full Text
Mechanism of neem limonoids-induced cell death in cancer: Role of oxidative phosphorylation: N. Yadav, et al.; Free Radic. Biol. Med. 90, 261 (2016), Application(s): Quantification of apoptosis and caspase activity measurement, Abstract
Insights into the mechanism of human papillomavirus E2-induced procaspase-8 activation and cell death: N. Singh, et al.; Sci. Rep. 6, 21408 (2016), Application(s): Caspase-8 activity assay, Abstract
Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells: Y. Pang, et al.; Exp. Cell Res. 16, 30161 (2016), Application(s): Fluorometric assay for caspase-8 activity, Abstract
Organ specific alteration in caspase expression and STK3 proteolysis during the aging process: M. Lessard-Beaudoin, et al.; Neurobiol. Aging 47, 50 (2016), Application(s): Caspase activity assays, Abstract — Full Text
Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro: F. Brisdelli, et al.; Life Sci. 158, 89 (2016), Application(s): Caspase activity, Abstract
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): Caspase activity detected in human neutophils, Abstract — Full Text

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Ac-IETD-AFC

ALX-260-110

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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