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Ac-IEPD-pNA

BML-P133

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Caspase substrate

Colorimetric granzyme B substrate. Combinatorial and kinetic studies have found the IEPD sequence to be optimal for cleavage of a tetrapeptide substrate by recombinant granzyme B, with a K_m for Ac-IEPD-pNA of 57 µM. Cleavage of the substrate by granzyme B releases pNA, which can be detected by its absorbance at 405 nm.

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Product Details

Alternative Name	Caspase-8 substrate (chromogenic), Granzyme B substrate (chromogenic)
Appearance	Lyophilized solid.
Formula	C₂₈H₃₈N₆O₁₁
MW	634.7
Purity	≥95%
Sequence	N-acetyl-Ile-Glu-Pro-Asp-pNA (p-nitroanilide)
Solubility	Soluble to at least 100mM in DMSO.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Stock solution in DMSO (e.g. 0.1M) is highly stable at -20°C or -70°C.
Handling	Keep cool and dry.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Reduced serpinB9-mediated caspase-1 inhibition can contribute to autoinflammatory disease: R. van der Burgh, et al.; Oncotarget 7, 19265 (2016), Abstract — Full Text
Approaches to design non-covalent inhibitors for human granzyme B (hGrB): M. Kim, et al.; Org. Biomol. Chem. 12, 8952 (2014), Abstract
Definition and redesign of the extended substrate specificity of granzyme B: J.L. Harris, et al.; J. Biol. Chem. 273, 27364 (1998), Abstract
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract

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Ac-IEPD-pNA

BML-P133

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

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