Ac-DEVD-AFC

Manuals And Inserts

• Datasheet

Specific Protocol

HEPES-Buffer (2x): 40 mM HEPES, pH 7.5, 20% glycerol, 4 mM DTT. Dilute to 1x with sterile distilled water prior to use.
Substrate: Prepare 20 mM stock solution in DMSO

- Induce apoptosis and prepare cell lysate or use recombinant caspase.
- Prepare reaction buffer: 10 µl of substrate stock solution + 1 ml 1x HEPES-Buffer for each reaction.
- Add an appropriate amount of cell lysate
(50-100 µl; should be titrated) or recombinant caspase to reaction buffer.
- Incubate for 1 hour at 37 °C.
- Measure with spectrofluorometer: 400 nm excitation wavelength, 505 nm emission wavelength.
- Suggested controls:
- Reaction mixture without substrate.
- Reaction mixture with non-apoptotic cell lysate.
- Reaction mixture with apoptotic cell lysate and caspase inhibitor.

Certificate of Analysis

Please enter the lot number as featured on the product label

Lot No. :

SDS

Enzo Life Science provides GHS Compliant SDS

• German (EU) SDS
• French (EU) SDS
• English (EU) SDS

If your language is not available please fill out the SDS request form