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6-keto-PGF_1α ELISA kit

ADI-901-004

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Highly sensitive ELISA, detecting as low as 1.40 pg/ml in just 3 hours.

Fast results from up to 38 samples in duplicate in just 3 hours
Highly specific with low cross-reactivity with structurally related molecules
Reliable and fully validated in-house with a variety of matrices
Widely published in peer-reviewed journals
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The 6-keto-PGF_1α EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This is a highly specific kit with low cross-reactivity to structurally related molecules. The 6-keto-PGF_1α EIA kit has been fully validated in-house in a wide variety of sample matrices.

6-keto-PGF_1α is a stable hydrolysis product of prostacyclin (PGI₂). Due to the short half-life of PGI₂ in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF_1α metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF_1α. PGI₂ has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.

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Product Details

Alternative Name	6-keto Prostaglandin F1α
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of 6-keto-PGF_1α in culture supernatants, serum, saliva, and urine from any species.
Assay Time	<3 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	DxS Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Sensitivity	1.40 pg/ml (range 3.2 – 50,000 pg/ml)
Species Reactivity	Species independent
Wavelength	405 nm

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Molecular genomic and epigenomic characteristics related to aspirin and clopidogrel resistance: Kim, J., Shin, B. S., et al.; BMC Med. Genomics 17, 166 (2024), Sample Type: Human plasma, Abstract
Vascular lipid droplets formed in response to TNF, hypoxia, or OA: biochemical composition and prostacyclin generation: M.Z. Pacia, et al.; J. Lipid Res. 64, 100355 (2023), Sample Type: Mouse culture supernatant (aortic ring culture), Abstract
Laser Speckle Contrast Imaging-based diagnosis of severe mesenteric traction syndrome: Hemodynamics and prostacyclin – A prospective cohort study: A.A. Olsen, et al.; Microvasc. Res. 147, 104505 (2023), Sample Type: Human plasma, Abstract
Dietary sodium restriction prevents vascular endothelial growth factor inhibitor-induced hypertension: L. van Doorn, et al.; Br. J. Cancer 128, 354 (2023), Sample Type: Human plasma, Abstract
Contribution of cyclooxygenase-1-dependent prostacyclin synthesis to bradykinin-induced dermal extravasation: M. Krybus, et al.; Biomed. Pharmacother. 148, 112786 (2022), Sample Type: tissue lysate (Mouse) and culture supernatant (Human foreskin-derived dermal microvascular endothelial cells ), Abstract
Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage: K.M. Mirabito Colafella, et al.; Clin. Sci. (Lond.) 136, 675 (2022), Sample Type: Rat plasma and urine, Abstract
Effect of the Renin-Angiotensin-Aldosterone System Reactivity on Endothelial Function and Modulative Role of Valsartan in Male Subjects with Essential Hypertension: Jasiczek, J., Trocha, M., et al.; J. Clin. Med. 10, 5816 (2021), Sample Type: Human plasma, Abstract — Full Text
Estrogen modulates metabolic risk profile after resistance training in early postmenopausal women: a randomized controlled trial: T.V. Dam, et al.; Menopause 28, 1214 (2021), Abstract
Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum: G. Mustafa, et al.; Prostaglandins Other Lipid Mediat. 155, 106565 (2021), Sample Type: Rat tissue lysate (Intestine [terminal ileum}), Abstract
Laminar shear stress upregulates the expression of PPARs in vascular endothelial cells under high free fatty acid-induced stress: Wang, Y. L., Chen, C. T., et al.; Exp. Ther. Med. 21, 438 (2021), Sample Type: Human culture supernatant (aourtic endothelial cells), Abstract