6-keto-PGF_1α ELISA kit

• Fast results from up to 38 samples in duplicate in just 3 hours
• Highly specific with low cross-reactivity with structurally related molecules
• Reliable and fully validated in-house with a variety of matrices
• Widely published in peer-reviewed journals

The 6-keto-PGF_1α EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This is a highly specific kit with low cross-reactivity to structurally related molecules. The 6-keto-PGF_1α EIA kit has been fully validated in-house in a wide variety of sample matrices.

6-keto-PGF_1α is a stable hydrolysis product of prostacyclin (PGI₂). Due to the short half-life of PGI₂ in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF_1α metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF_1α. PGI₂ has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.