The acetoxymethyl group of 6-Bnz-cAMP-AM masks the charged polar phosphate and thus makes the molecule highly membrane-permeant. Inside the cell esterases release the more polar 6-Bnz-cAMP which is a potent, site-selective activator of cAMP-dependent protein kinase. 6-Bnz-cAMP does not activate Epac and thus can be used as an Epac-negative control.
Phosphate tris(acetoxymethyl)ester, PO4-AM3 (Prod. No. BLG-P030), is recommended as control reagent in 6-Bnz-cAMP-AM applications to test for side effects of enzymatically released acetic acid and formaldehyde, two metabolites with potential biological functions.
BLG-B079-05 (5 x 1 µmol pack size) is not sold in the U.S. or Canada. Please contact us for available options.
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