5-FITC (ultra pure)

Fluorescein isothiocyanate (FITC) is an amine-reactive fluorescein derivative that yields conjugates with superior stability. Dye synthesis generally results in two isomers that differ slightly in protein binding and may elute differently under chromatographic conditions, complicating analysis in certain situations. The 5-isomer of FITC is the most widely used isoform and has a multitude of applications beyond protein peptide and nucleotide labeling. For example, it is commonly employed as a donor for FRET (Fluorescence Resonance Energy Transfer) based assay systems. An acceptor molecule, such as TRITC, is recommended in such FRET applications. Wavelength Maxima: Excitation 494nm, Emission 520nm